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Biocompatible photolithographic process for the patterning of biomolecules
Antonios Douvas1, Panagiotis Argitis, Konstantinos Misiakos
1Institute of Microelectronics, NCSR Demokritos, 15310, Aghia Paraskevi, Greece.
Biosensors & Bioelectronics
|February 19, 2002
Summary
A novel photoresist enables biocompatible patterning of biomolecules for immunoassays. This technique achieves high resolution on various surfaces, including capillary interiors, using gentle processing conditions.
Area of Science:
- Biomaterials Science
- Photolithography
- Surface Chemistry
Background:
- Biomolecule patterning is crucial for developing advanced immunoassays and biosensors.
- Existing lithographic techniques often employ harsh chemicals or conditions incompatible with sensitive biomolecules.
- There is a need for biocompatible patterning methods that maintain biomolecule integrity.
Purpose of the Study:
- To introduce a new photoresist material and process for biocompatible biomolecule patterning.
- To demonstrate the lithographic capabilities and resolution of the developed photoresist under mild conditions.
- To validate the applicability of the technique on diverse substrates, including microfluidic devices.
Main Methods:
- Development of a photoresist based on poly(t-butyl acrylate) for positive imaging.
- Utilizing a sulfonium salt photoacid generator for sensitivity at lambda>300 nm.
- Employing dilute basic solutions and biomolecule-compatible thermal processing steps.
- Applying the lithographic process to silicon wafer substrates and polystyrene capillary surfaces.
Main Results:
- Achieved resolution of 5-10 microm for equal lines/spaces on silicon wafers under biocompatible conditions.
- Demonstrated successful patterning on substrates with complex geometries, such as the inner surfaces of capillaries.
- Successfully patterned mouse IgG onto the inner surface of a polystyrene capillary, showcasing the process's utility.
Conclusions:
- The developed poly(t-butyl acrylate)-based photoresist offers a viable solution for biocompatible micro- and nanofabrication of biomolecule layers.
- The lithographic process is suitable for patterning sensitive biomolecules under conditions that preserve their functionality.
- The technique's adaptability to various substrates, including capillary surfaces, opens avenues for novel biosensor and diagnostic device development.