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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Advances in proteome analysis by mass spectrometry
T J Griffin1, D R Goodlett, R Aebersold
1Institute for Systems Biology, 4225 Roosevelt Way NE, Suite 200, Seattle, WA 98105, USA. tgriffin@systemsbiology.org
Current Opinion in Biotechnology
|February 19, 2002
Summary
Mass spectrometry enables systematic biological investigation and gene function studies. Advances focus on separation, labeling, modification analysis, and instrumentation for proteome characterization.
Area of Science:
- Biochemistry and Molecular Biology
- Analytical Chemistry
Background:
- Proteome characterization is crucial for understanding biological systems and gene function.
- Mass spectrometry is a key technology in this field.
Purpose of the Study:
- To review recent advancements in mass spectrometry-based proteome characterization.
- To highlight key areas of progress in the field.
Main Methods:
- Review of recent literature and technological developments.
- Focus on four key areas: separation, labeling, post-translational modifications, and instrumentation.
Main Results:
- Significant progress has been made in protein and peptide separation techniques.
- New selective labeling chemistries allow for quantitative proteomic measurements.
- Improved methods for characterizing post-translational protein modifications are available.
- Advancements in mass spectrometry instrumentation enhance proteome analysis.
Conclusions:
- Recent innovations in mass spectrometry have significantly advanced proteome characterization.
- These advancements facilitate a more systematic investigation of biological systems and gene function.
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