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[Analysis on the genetics of Hantavirus S85-46 isolated from Sichuan province]
1National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Objective:
To understand the molecular and epidemic characteristics of Hantavirus S85-46 isolated from Sichuan province.
Methods:
RT-PCR products were amplified from S85-46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
Results:
The homology of M segment of S85-46 strain and HTN type strain varied from 84.1%-99.7%, compared to 70.4%-70.9% with SEO strains. This result indicated that S85-46 strain belonged to hantavirus. The nucleotide sequence analyses indicated that S85-46 strain, LR1 strain and 76-118 strain were highly homologous. The two latter strains were isolated from Shaanxi province of China and South Korea respectively, which were far from Sichuan province of China. The nucleotide sequence analyses of S segment was similar to M segment.
Conclusion:
The epidemic strains isolated from different regions may have been highly homologous.
