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Updated: Aug 1, 2026

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
Regulation of glucose transporter 1 expression by gliclazide in rat L6 myoblasts
H Imamur1, I Morimoto, Y Tanaka
1Department of Internal Medicine, Niigata Rosai Hospital, Japan. 630tanuki128-826@jcom.home.ne.jp
Abstract:
Sulphonylureas are known to enhance insulin secretion from the pancreas and its sensitivity of the extrapancreatic target organs. In this study, we clarified a direct extrapancreatic effect of the sulphonylureas and of gliclazide, on the glucose transport system in cultured rat L6 myoblasts, which predominantly expressed glucose transporter 1 (GLUT 1). Our results show that gliclazide stimulated 2-deoxy-[3H]-D-glucose (2DG) uptake, 24 h after treatment, in a dose-dependent manner, and it also increased GLUT 1 protein synthesis and mRNA expression; 2DG uptake and GLUT 1 protein synthesis induced by gliclazide were completely blocked by protein kinase A (PKA) inhibitors (H89 and rp-cAMP), and gliclazide increased the intracellular cAMP levels 3 to 24 hr after the treatment. These results show that in rat L6 myoblasts, gliclazide stimulates glucose transport activity by the induction of GLUT 1 gene expression through PKA.
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