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Updated: Oct 2, 2026
Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK
Published on: December 18, 2012
[An experimental study of immune mediating action of T-lymphocyte subsets on recurrent herpetic stromal keratitis]
1Department of Ophthalmology, The Second Affiliated Hospital, China Medical University, Shenyang 110003, China.
Objective:
To investigate the role of T-lymphocyte subsets in the recurrent herpetic stromal keratitis (SK).
Methods:
In a recurrent SK NIH mouse model, rat monoclonal antibodies were used to selectively deplete in vivo the CD(+)(4) and CD(+)(8) T-lymphocytes in mice, and the effects of the subsets on recurrent herpetic SK were evaluated.
Results:
As measured by flow cytometry, in the mice treated with GK1.5, > 90% of CD(+)(4) T cells were depleted; and in the mice treated with H-35, > 95% of CD(+)(8) T cells were depleted. Depletion of CD(+)(4) T cells could halt the recurrence of SK, reduce the severity of recurrent SK and impair the delayed type hypersensitivity. In contrast, depletion of CD(+)(8) T cells had no effect on the outcome of the disease. Histopathologic examination of the recurrent SK demonstrated that the stromal inflammation in CD(+)(8) T-cell depleted and control mice was characterized by extensive lymphocyte and polymorphonuclear leukocyte infiltration, but only occasional lymphocytes were observed in CD(+)(4) T-cell depleted mice.
Conclusion:
The results provide evidence that recurrent herpetic SK represents an immunopathologic process mediated by CD(+)(4) T cells.
