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[Experimental study on herpes simplex virus-1 functional gene latency in cornea].

L Xie1, S Li, X Dong

  • 1Institute of Ophthalmology and Eye Hospital, Shandong Academy of Medical Sciences, Qingdao 266071, China. lixinxie@public.qd.sd.cn

[Zhonghua Yan Ke Za Zhi] Chinese Journal of Ophthalmology
|February 21, 2002
PubMed
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Herpes simplex virus-1 (HSV-1) functional genes, including LAT and TK, persist in latently infected rabbit corneas. This suggests active viral gene retention during latency, impacting ocular health.

Area of Science:

  • Ophthalmology
  • Virology
  • Molecular Biology

Context:

  • Herpes simplex keratitis (HSK) is a significant cause of corneal blindness.
  • Understanding herpes simplex virus-1 (HSV-1) latency is crucial for managing recurrent HSK.
  • Previous research has focused on viral DNA presence, but functional gene expression during latency requires further investigation.

Purpose:

  • To investigate the presence and potential functionality of HSV-1 genes within latently infected corneal tissues.
  • To determine if key HSV-1 genes, such as latency-associated transcript (LAT), thymidine kinase (TK), and DNA polymerase, are actively retained during the latent phase of infection in the cornea.

Summary:

  • A rabbit model of HSK was established, and latently infected corneas were transplanted into naive rabbits.

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  • Corneal tissues were analyzed for HSV-1 antigen, LAT, TK, and DNA polymerase gene expression using PCR and antigen detection assays.
  • Results indicated that while HSV-1 antigen was not always detected, LAT, TK, and DNA polymerase genes were present in a significant proportion of latently infected corneal samples.

    • Impact:

    • This study provides evidence that functional HSV-1 genes are retained in the cornea during latency.
    • Findings suggest that viral gene expression, not just the presence of viral DNA, may play a role in maintaining HSV-1 latency in ocular tissues.
    • This deeper understanding of HSV-1 corneal latency could inform new therapeutic strategies for preventing HSK recurrence.