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Preliminary research on myosin light chain kinase in rabbit liver
1Department of Biochemistry and Molecular Biology, University of Science and Technology of China, Hefei 230027, Anhui Province, China.
Aim:
To study preliminarily the properties of myosin light chain kinase (MLCK) in rabbit liver.
Methods:
The expression of MLCK was detected by reverse transcription-polymerase chain reaction(RT-PCR); the MLCK was obtained from rabbit liver, and its activity was analyzed by gamma-(32)P incorporation technique to detect the phosphorylation of myosin light chain.
Results:
MLCK was expressed in rabbit liver, and the activity of the enzyme was similar to rabbit smooth muscle MLCK, and calmodulin-dependent. When the concentration was 0.65 mg x L(-1), the activity was at the highest level.
Conclusion:
MLCK expressed in rabbit liver may catalyze the phosphorylation of myosin light chain, which may play important roles in the regulation of hepatic cell functions.