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Interaction of PRMT1 with BTG/TOB proteins in cell signalling: molecular analysis and functional aspects
Cyril Berthet1, Fabienne Guéhenneux, Valérie Revol
1Unité INSERM U453, affiliée au CNRS, Centre Léon Bérard, Le Cheney, 28 rue Laënnec, 69373 Lyon Cedex 08, France.
Background:
Several recent reports have connected protein methylation with differentiation. Furthermore, the BTG/TOB proteins have also been implicated in such control. BTG1 and 2 have been shown to interact with PRMT1 (predominant cellular arginine N-methyltransferase of type I).
Results:
First, we have studied the interaction between PRMT1 and the proteins of the BTG/TOB family. We show that boxC, a sequence present only in BTG1 and BTG2, is essential for this association. Using boxC peptide, we have investigated the importance of PRMT1/BTG protein association during type I protein methylation reactions. Finally, we show that the addition of boxC fused to penetratin interferes with the neuronal differentiation of PC12 cells and ES cell-derived neurones.
Conclusions:
Taken together, these results indicate that PRMT1/BTG proteins could play a key role in the arginine methylation-mediated signalling pathway as well as in neuronal differentiation.