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Related Experiment Videos

A new fluid distribution system for scale-flexible expanded bed adsorption.

Jürgen J Hubbuch1, Anders Heebøll-Nielsen, Timothy J Hobley

  • 1Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, Søltofts Plads, DK-2800, Kgs. Lyngby, Denmark.

Biotechnology and Bioengineering
|February 22, 2002
PubMed
Summary

A novel rotating distributor enhances expanded bed adsorption by eliminating traditional components. Optimal performance, with minimal dead zones and efficient flow, was achieved at 2.5 rpm, demonstrating improved fluid dynamics for adsorption processes.

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Area of Science:

  • Chemical Engineering
  • Separation Science

Background:

  • Expanded bed adsorption (EBA) is a chromatographic technique used for direct capture of biological molecules.
  • Traditional EBA systems often rely on perforated plates or meshes, which can cause channeling and dead zones.
  • A new fluid distribution system using a rotating distributor was developed to improve flow dynamics in EBA columns.

Purpose of the Study:

  • To introduce and evaluate a novel rotating fluid distribution system for expanded bed adsorption.
  • To investigate the effect of distributor rotation rate on column hydrodynamics and performance.
  • To determine optimal operating conditions for the new distributor in an EBA column.

Main Methods:

  • A 150-cm diameter column was used with a 30-cm high bed of supports fluidized by tap water.

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  • Dye and acetone tracers were introduced to study flow patterns and residence time distribution.
  • Superficial fluid velocities and distributor rotation rates were varied to assess column performance.

    • Main Results:

    • Linear bed expansion was observed with increasing superficial fluid velocity (170-450 cm/h).
    • Distributor rotation rate had minimal impact on bed expansion (2.5-10 rpm).
    • Optimal flow pattern for EBA was achieved at 283 cm/h superficial velocity and 2.5 rpm rotation, showing no dead zones and a discrete tracer band.
    • Parabolic flow profile was indicated with interstitial velocities of 460 cm/h (wall) and 572 cm/h (center).
    • At optimal conditions (2.5 rpm, 283 cm/h), axial dispersion coefficient was 6.1 x 10(-6) m²/s with 29 theoretical plates.
    • Higher rotation rate (10 rpm) increased axial dispersion (8.08 x 10(-6) m²/s) and reduced theoretical plates (22).

    Conclusions:

    • The rotating distributor effectively generates a suitable flow pattern for expanded bed adsorption, eliminating dead zones.
    • An optimal operating condition of 2.5 rpm distributor rotation at 283 cm/h superficial fluid velocity was identified for efficient EBA.
    • This new system offers improved hydrodynamics and separation efficiency compared to conventional methods.