Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recommendations for sentinel lymph node processing in breast cancer.

M A Yared1, L P Middleton, T L Smith

  • 1Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston, USA.

The American Journal of Surgical Pathology
|February 23, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated blood volume estimation in surgical drains for clinical decision support.

European review for medical and pharmacological sciences·2024
Same author

Association between Fiber Intake and Risk of Incident Chronic Kidney Disease: The UK Biobank Study.

The journal of nutrition, health & aging·2023
Same author

TB in ageing populations: lessons from Japan and Korea.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2023
Same author

Comparing the effectiveness of prolotherapy and percutaneous dry needling in the treatment of lateral epicondylitis: a retrospective cohort study.

European review for medical and pharmacological sciences·2023
Same author

Sous-vide cooking as a practical strategy to improve quality attributes and shelf stability of reduced-salt chicken breast ham.

Poultry science·2023
Same author

Consumer Trend of Using Ready-to-Use Salted Napa Cabbage at Home: Current Consumer Behaviors, Beliefs, and Opinions about the Main Ingredient of Kimchi.

Journal of food protection·2022
Same journal

Clinicopathologic and Molecular Analysis of Traditional Serrated Adenoma and Its Possible Precursor Lesions With Special Reference to RSPO Fusion Status: A Large Case Series From Single Institution.

The American journal of surgical pathology·2026
Same journal

NF2/Merlin Immunohistochemistry Helps Distinguish Collecting Duct Carcinoma From Upper Tract Urothelial Carcinoma.

The American journal of surgical pathology·2026
Same journal

Neuroendocrine Carcinoma of the Gallbladder: Clinicopathologic and Immunohistochemical Analysis of 31 Cases.

The American journal of surgical pathology·2026
Same journal

Papillary Renal Neoplasm With Reverse Polarity Is a Distinct Distal Nephron-Derived Tumor With Unique Methylation Profile.

The American journal of surgical pathology·2026
Same journal

Biallelic ARID1A Alterations: A Promising Novel Biomarker for Risk Stratification and Management in Pediatric Malignant Hepatocellular Tumors.

The American journal of surgical pathology·2026
Same journal

Absent Cyclin D1 Expression in Myeloid Sarcomas Distinguishes From Malignant Histiocytic Neoplasms: When Morphologic Ambiguity is Deceptive.

The American journal of surgical pathology·2026
See all related articles

Sentinel lymph node (SLN) biopsy is crucial for breast cancer staging. Examining specific stained levels of SLNs improves metastasis detection, aiding accurate axillary staging.

Area of Science:

  • Oncology
  • Surgical Pathology

Background:

  • Sentinel lymph node (SLN) status is a key indicator for axillary staging in breast cancer patients.
  • Accurate SLN evaluation is critical for determining the need for further axillary treatment.

Purpose of the Study:

  • To optimize the histological processing protocol for sentinel lymph nodes (SLNs) in breast cancer.
  • To identify the most effective method for detecting micrometastases in SLNs.

Main Methods:

  • 173 SLNs from 96 breast cancer patients were analyzed.
  • Serial sectioning of lymph nodes into 10 levels with Hematoxylin and Eosin (H&E) and Pan-cytokeratin staining.
  • Comparison of metastasis detection rates across different staining levels and methods.

Main Results:

Related Experiment Videos

  • Metastases were identified in 22 SLNs (19 patients) upon examination of all 10 levels.
  • Initial H&E or cytokeratin levels detected metastases in 95.5% of positive SLNs.
  • A protocol of initial H&E, two additional H&E, and one cytokeratin section identified node status in 97.9% of patients.

Conclusions:

  • A recommended protocol involves initial H&E staining, followed by two additional H&E and one cytokeratin-stained section for comprehensive SLN evaluation.
  • This optimized protocol enhances the accuracy of detecting axillary metastases in breast cancer patients.
  • Improved SLN processing can refine staging and guide treatment decisions for breast cancer.