Related Experiment Videos
A competitive immunoassay to detect a hapten using an enzyme-labelled peptide mimotope as tracer
F Sellrie1, J A Schenk, O Behrsing
1Institute of Biochemistry and Biology, Biotechnology, Potsdam University, Karl-Liebknecht-Str. 24-25, 14476 Golm, Germany.
Journal of Immunological Methods
|February 28, 2002
Abstract:
Mimotope peptides-peptides which mimic the binding of a hapten to its corresponding monoclonal antibody-were conjugated to peroxidase and used in competitive immunoassay. The established immunoassay was used to quantitatively determine the concentration of hapten. As model system in all the experiments described here, we used the binding of the monoclonal antibody B13-DE1 to fluorescein and the corresponding peptide mimotope.