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Patterns of microbial colonization in primary root canal infections
José F Siqueira1, Isabela N Rôças, Hélio P Lopes
1Department of Endodontics, School of Dentitry, EstáCio de Sá University, Rio de Janeiro, Brazil. siqueira@estacio.br
Summary
Microbial infections heavily colonize root canals in teeth with periradicular lesions, penetrating dentinal tubules. This extensive root canal infection, including bacteria and fungi, necessitates thorough therapeutic measures for complete elimination.
Area of Science:
- Microbiology
- Endodontics
- Scanning Electron Microscopy
Background:
- Chronic periradicular lesions often indicate underlying root canal infection.
- Understanding microbial colonization patterns is crucial for effective treatment.
Purpose of the Study:
- To investigate microbial infection patterns in root canals of teeth with chronic periradicular lesions.
- To utilize scanning electron microscopy (SEM) for detailed visualization of root canal colonization.
Main Methods:
- Fifteen extracted teeth with carious and periradicular lesions were selected.
- Teeth were processed, split, dehydrated, gold-coated, and examined using SEM.
- SEM was employed to visualize and analyze microbial colonization within the root canal system.
Main Results:
- All root canals examined showed evidence of infection, with bacteria present throughout.
- Colonization patterns varied, with cocci and rods forming mixed communities on canal walls.
- Bacteria were observed penetrating dentinal tubules, reaching up to 300 micrometers in depth; yeast was seen in one case.
Conclusions:
- Root canals associated with periradicular lesions are heavily infected by bacteria and sometimes fungi.
- The observed climax community pattern of root canal microbiota may require specialized treatment approaches.
- Complete elimination of root canal infection is essential, suggesting the need for comprehensive therapeutic strategies.