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Updated: Aug 14, 2026
Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
Published on: September 12, 2014
[Primary research about reconstruction of cornea in three-dimensional collagen gel in vitro]
1Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Objective:
To research the reconstruction of cornea in three-dimensional culture.
Methods:
Using primary cultured corneal epithelial cells, stromal cells and endothelial cells rebuilt a corneal structure in three-dimensional collagen gel reconstruction system. The artificial corneal transparency was examined by a slit-lamp microscope. The biological characters of cells were tested by routine pathologic and transmission electron microscopic technology.
Results:
The reconstructed corneal tissue formed three layers. The epithelial cell layer was two or three cell thickness. The endothelial cells formed a single layer. The stromal cells were diffuse in distribution in the collagen gel. The cells had proliferative ability and capability of producing collagen.
Conclusion:
The regular and transparent corneal structure can be rebuilt by tissue engineering technology.
