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Updated: Aug 4, 2026
Experimental Metastasis Assay
Published on: August 24, 2010
[Zymographic analysis of matrix metalloproteinases in mouse carcinoma cell lines with different lymphatic metastasis
Objective:
To investigate the correlation between lymphatic metastasis and matrix metalloproteinases (MMPs) activity.
Methods:
Zymographic analysis was used to detect the difference of MMPs product and activities between high lymphatic metastatic cell line (HCa-F) and low lymphatic metastatic cell line (HCa-P), that were cultured in the medium including homogenate of lymph node or liver or spleen tissue.
Results:
HCa-F and HCa-P cells produced small quantities of MMP-9 in 1640 medium. After adding lymph node homogenate to the culture medium, F cells produced a large quantity of MMP-9 as well as active MMP-9 and MMP-2. P cells also produced MMP-9, active MMP-9 and MMP-2, but were much lower than that of F cells. Neither F cells nor P cells produced MMPs in the media with homogenate of liver or spleen added.
Conclusion:
The metastatic potential of F cells is stronger than that of P cells may be due to F cell's ability to secrete MMPs is stronger than P cell's, therefore the production of MMPs of hepatocarcinoma cells is closely correlated to their metastatic potential.
