Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cortical spreading depression transiently activates MAP kinases.

Ava K Chow1, C S Thompson, Matthew J Hogan

  • 1Neuroscience Research Institute, Faculty of Medicine, University of Ottawa, 451 Smyth, Ontario, Ottawa, Canada K1H 8M5.

Brain Research. Molecular Brain Research
|March 1, 2002
PubMed
Summary

Cortical spreading depression (CSD) offers neuroprotection before brain ischemia. CSD activates the MAPK pathway, specifically ERK phosphorylation, which may explain its protective mechanism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EdTech Cache: A database of interactive learning content for dental education and beyond.

Canadian journal of dental hygiene : CJDH = Journal canadien de l'hygiene dentaire : JCHD·2026
Same author

Metaverse in dental hygiene education: Are we prepared for the future?

Canadian journal of dental hygiene : CJDH = Journal canadien de l'hygiene dentaire : JCHD·2026
Same author

<i>Save Our School</i>: an educational game made with Gimkit Creative.

Canadian medical education journal·2026
Same author

Featuring the application of biochemistry in dental practice through a self-directed assignment: A descriptive study.

PloS one·2026
Same author

Quiz Game Show With PowerPoint and Zoom: Revitalizing a Classical Tool for Modern Learners.

Journal of dental education·2026
Same author

Games as homework to promote student engagement in an asynchronous online course.

Canadian journal of dental hygiene : CJDH = Journal canadien de l'hygiene dentaire : JCHD·2026

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Ischemic Preconditioning

Background:

  • Cortical spreading depression (CSD) demonstrates neuroprotective potential against cerebral ischemia.
  • The underlying mechanisms of CSD-induced neuroprotection are not fully understood.
  • Mitogen-activated protein (MAP) kinases are implicated in neuroprotection during ischemic preconditioning.

Purpose of the Study:

  • To investigate the role of MAP kinase (MAPK) pathways in mediating the neuroprotective effects of CSD.
  • To determine if CSD activates MAPK signaling, specifically the ERK pathway.

Main Methods:

  • Induction of cortical spreading depression (CSD) in a rodent model.
  • Analysis of extracellular signal-regulated kinase (ERK) and MEK phosphorylation via Western blotting and immunohistochemistry.

Related Experiment Videos

  • Assessment of the temporal dynamics of MAPK activation post-CSD.

    • Main Results:

    • CSD significantly increased ERK phosphorylation in a MEK-dependent manner.
    • This ERK phosphorylation was transient, returning to baseline levels within 45 minutes.
    • Immunohistochemistry showed ubiquitous ERK phosphorylation in cortical cells, while MEK phosphorylation was localized to the nucleus.

    Conclusions:

    • CSD activates the MAPK pathway, involving MEK and ERK phosphorylation.
    • The transient nature and cellular localization of MAPK activation suggest a role in CSD-mediated preconditioning.
    • These findings provide insight into the molecular mechanisms underlying CSD's neuroprotective effects.