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Related Experiment Videos

Incomplete retention after direct myocardial injection.

P Michael Grossman1, Zhenguo Han, Maria Palasis

  • 1Division of Cardiology, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, Michigan, USA.

Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions
|March 1, 2002
PubMed
Summary

Direct intramyocardial injection for heart disease shows variable retention. Catheter-based delivery retains more agents than epicardial injection, with smaller volumes improving retention.

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Area of Science:

  • Cardiovascular Medicine
  • Biomedical Engineering
  • Regenerative Medicine

Background:

  • Direct intramyocardial injection offers potential for localized delivery of therapeutic agents for myocardial and coronary artery disease.
  • Limited data exists on the immediate fate of agents delivered via percutaneous endomyocardial catheters or surgical epicardial injection.

Purpose of the Study:

  • To evaluate acute retention of agents injected directly into the myocardium using a novel method.
  • To compare epicardial versus percutaneous endocardial and postmortem delivery of injectates.
  • To assess the influence of injectate volume on myocardial retention.

Main Methods:

  • Utilized neutron-activated microspheres as tracers in pigs for acute retention studies.
  • Compared percutaneous endomyocardial, open-chest epicardial, and postmortem epicardial injection methods.

Related Experiment Videos

  • Assessed viral transfection efficiency (adenovirus encoding lac-Z) with varying injectate volumes in a separate study.

    • Main Results:

    • Endomyocardial injection demonstrated significantly higher microsphere retention (43% ± 15%) compared to open-chest epicardial injection (15% ± 21%).
    • Postmortem injection showed the highest retention (89% ± 60%), while reducing injectate volume from 100 to 10 microL improved microsphere retention (P=0.01).
    • A trend towards improved viral transfection was observed with smaller injection volumes.

    Conclusions:

    • Significant injectate loss occurs despite direct intramyocardial administration, potentially due to channel leakage and vascular/lymphatic return.
    • Catheter-based endomyocardial injection offers comparable or superior injectate retention versus open-chest epicardial delivery.
    • Lower injection volumes may enhance myocardial retention of therapeutic agents.