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Aspartame optical biosensor with bienzyme-immobilized eggshell membrane and oxygen-sensitive optode membrane
1College of Chemistry and Chemical Engineering, Hunan University, Changsha, PR China.
A novel aspartame optical biosensor uses a bienzyme system immobilized on eggshell membrane for accurate detection. This stable biosensor offers a wide working range and minimal interference, enabling aspartame quantification in commercial products.
Area of Science:
- Biotechnology
- Biosensor Technology
- Analytical Chemistry
Background:
- Aspartame detection is crucial for food quality control.
- Existing methods for aspartame analysis can be complex or time-consuming.
- Development of sensitive and stable biosensors is needed for efficient aspartame monitoring.
Purpose of the Study:
- To fabricate a novel optical biosensor for aspartame detection.
- To utilize a bienzyme system immobilized on an eggshell membrane.
- To evaluate the performance and stability of the developed aspartame biosensor.
Main Methods:
- Immobilization of alpha-chymotrypsin and alcohol oxidase onto eggshell membrane.
- Utilizing an oxygen-sensitive optode membrane as a transducer.
- Monitoring oxygen depletion and fluorescence enhancement for aspartame quantification.
Main Results:
- The biosensor demonstrated excellent stability with an 8-month shelf life.
- A linear relationship was observed between fluorescence change rate and aspartame concentration.
- The working range was established from 0.056 to 3.07 mM with high specificity, excluding common interferences except ethanol.
Conclusions:
- The developed aspartame optical biosensor is stable, sensitive, and specific.
- The eggshell membrane immobilization technique offers advantages for biosensor fabrication.
- The biosensor is suitable for determining aspartame content in commercial products.
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