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Demecolcin and vinblastine sulphate as stathmokinetic agents for different tissues of the hairless mouse
Abstract:
The dose/response relationship of the kinetostatic effect of demecolcin (ColcemidR) and vinblastine sulphate (VelbeR) was estimated in the epidermis, the forestomach, the jejunum and the colon of the hairless mouse (hr/hr) at 3 hours after intraperitoneal (i.p.) injection. A dose of 0.15 mg Colcemid per animal was found to be the lowest effective dose in all the organs tested. The value did not differ much from the corresponding effective dose of Velbe. This dose of Colcemid was then used to study the accumulation of arrested mitoses during the first 4 hours after i.p. injection. An almost linear increase in the mitotic count was found up to 4 hours in the epidermis, 3.5 hours in the forestomach and 3 hours in the jejunum and the colon. A dose of 0.15 mg Colcemid per animal i.p. with an observation time of 3 hours is thus a reliable method for estimation of the mitotic rate in many organs. It is also concluded that both Colcemid and Velbe can be used as reliable stathmokinetic drugs for both epidermis, forestomach and jejunum and colon mucosa provided that correct doses and short enough accumulation time (3 hours) is used.
Insights
Demecolcine and vinblastine sulfate are effective stathmokinetic drugs for assessing mitotic rates in mouse tissues. A 0.15 mg dose of demecolcine per animal is reliable for estimating mitotic activity across various organs.
Area of Science:
- Pharmacology
- Cell Biology
- Toxicology
Background:
- Stathmokinetic drugs are crucial for cell cycle analysis.
- Understanding the dose-response relationship of these agents is vital for accurate mitotic rate estimation.
- Demecolcine and vinblastine sulfate are commonly used antimitotic agents.
Purpose of the Study:
- To determine the dose-response relationship of demecolcine and vinblastine sulfate.
- To establish reliable methods for estimating mitotic rates in various mouse tissues.
- To evaluate the efficacy of these drugs as stathmokinetic agents.
Main Methods:
- Hairless mice (hr/hr) were administered intraperitoneal injections of demecolcine or vinblastine sulfate.
- Dose-ranging studies were conducted to identify the lowest effective dose.
- Mitotic counts were assessed at various time points post-injection in epidermis, forestomach, jejunum, and colon.
- Accumulation of arrested mitoses was studied over a 4-hour period.
Main Results:
- A dose of 0.15 mg demecolcine per animal was the lowest effective dose across all tested organs.
- This effective dose was comparable to that of vinblastine sulfate.
- Mitotic counts increased nearly linearly up to 4 hours in the epidermis, 3.5 hours in the forestomach, and 3 hours in the jejunum and colon.
- A 3-hour observation period with 0.15 mg demecolcine per animal proved reliable for mitotic rate estimation.
Conclusions:
- Demecolcine and vinblastine sulfate are reliable stathmokinetic drugs for evaluating mitotic rates in mouse epidermis, forestomach, jejunum, and colon.
- An intraperitoneal dose of 0.15 mg demecolcine per animal with a 3-hour accumulation time is a robust method for mitotic rate assessment.
- Optimal drug dosage and short accumulation times are essential for accurate stathmokinetic analysis.