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A novel two-enzyme amperometric electrode for lactose determination
Handan Gülce1, Ahmet Gülce, Attila Yildiz
1Department of Chemistry, Suleyman Demirel University, Isparta, Turkey.
Summary
A novel enzyme electrode was developed for lactose determination by coimmobilizing beta-galactosidase and glucose oxidase in a redox polymer. This biosensor utilizes the electrooxidation of hydrogen peroxide for sensitive lactose analysis.
Area of Science:
- Biochemistry
- Electrochemistry
- Biosensor Technology
Background:
- Lactose determination is crucial in food and clinical analysis.
- Enzyme electrodes offer sensitive and selective analytical methods.
- Redox polymers provide a stable matrix for enzyme immobilization.
Purpose of the Study:
- To develop a novel enzyme electrode for lactose determination.
- To investigate the coimmobilization of beta-galactosidase and glucose oxidase.
- To optimize electrode performance for accurate analysis.
Main Methods:
- Coimmobilization of beta-galactosidase and glucose oxidase in polyvinylferrocenium perchlorate (PVF+ ClO4-).
- Amperometric detection at +0.70 V vs. SCE.
- Elucidation of substrate and buffer concentration effects, and pH influence.
Main Results:
- Successful development of a coimmobilized enzyme electrode.
- Amperometric response correlated with enzymatically produced H2O2.
- Optimized conditions for substrate/buffer concentrations and pH were identified.
Conclusions:
- The developed enzyme electrode is effective for lactose determination.
- The polyvinylferrocenium perchlorate matrix supports enzyme activity and electrochemical detection.
- Further optimization can enhance the biosensor's analytical performance.