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Related Concept Videos

Bacterial Phylum Cyanobacteria01:30

Bacterial Phylum Cyanobacteria

Cyanobacteria are a diverse group of oxygenic, phototrophic bacteria that played a pivotal role in converting Earth’s atmosphere from anoxic to oxygen-rich billions of years ago. They exhibit remarkable morphological diversity, ranging from unicellular forms to filamentous types, with cell sizes varying between 0.5 μm and 100 μm. Cyanobacteria are classified into five groups: Chroococcales (unicellular, dividing by binary fission), Pleurocapsales (unicellular, dividing by multiple fission),...

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Related Experiment Video

Updated: Jul 24, 2026

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
11:45

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

Published on: May 29, 2016

Genetic tools for cyanobacteria.

O A Koksharova1, C P Wolk

  • 1MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824, USA.

Applied Microbiology and Biotechnology
|March 6, 2002
PubMed
Summary

Cyanobacteria research benefits from advanced genetic tools and genomic sequencing. These resources accelerate the study of photosynthesis, nitrogen fixation, and evolution, enabling new biotechnological applications.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Cyanobacteria are oxygenic photosynthetic bacteria crucial for studying diverse biological processes.
  • Research encompasses photosynthesis, cell differentiation, N2 fixation, metabolism, stress resistance, and molecular evolution.

Purpose of the Study:

  • To highlight the increasing use of cyanobacteria in biological research.
  • To review available genetic tools and genomic data for cyanobacteria.
  • To discuss the implications for future research and biotechnological applications.

Main Methods:

  • Development of vectors and genetic tools for unicellular and filamentous cyanobacteria.
  • Gene transfer techniques including transformation, electroporation, and conjugation.

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Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria
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Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

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  • Mutagenesis methods for isolating specific mutants, including site-directed mutants.
  • Use of reporter genes for transcriptional analysis at colony and cellular levels.
  • Genomic sequencing of key cyanobacterial strains (e.g., Synechocystis sp. PCC 6803, Anabaena sp. PCC 7120).

    • Main Results:

    • Extensive genetic tools and methods are available for cyanobacteria research.
    • Complete genomic sequences for key strains are available, with ongoing projects for others.
    • Genomic data facilitates global monitoring of gene expression and accelerates gene analysis.
    • Evolutionary relationships, including chloroplast origins, can be studied.

    Conclusions:

    • The availability of genetic tools and genomic data significantly enhances cyanobacteria research.
    • These resources accelerate the understanding of fundamental biological processes and evolutionary history.
    • Cyanobacteria hold significant potential for future biotechnological applications.