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[Increased expression of peritoneal macrophage CD14 in severely burned mice]
1Burn Center, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
Objective:
To observe the dynamic changes in peritoneal macrophage (Mphi) CD14 expression in severely burned mice.
Methods:
The mice, being inflicted with 20% TBSA III degree burn, were randomly divided into two groups: Group A: The mice in this group received fluid resuscitation instantaneously after burn. Group B: The mice in this group received delayed fluid resuscitation at 6hr postburn. The Mphis at different postburn time points were obtained in two groups for the detection of their expression of CD14 and CD14 mRNA with immunohistochemical and RT -- PCR analysis, and also for the determination with ELISA of the changes in TNF and IL -- 6 levels in the supernatant of their culture.
Results:
The expression of CD14 and the levels of TNF and IL -- 6 increased obviously in both groups from 1 to 24 hrs postburn.
Conclusion:
Burn injury would induce the increase of the expression of Mphi CD14 mRNA in mice. As a result, the Mphi became more readily to be activated by low levels of LPS. The activated Mphi might play an important role in the development of postburn SIRS.