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[The study of low dose radiation inducing hormesis effect on hematopoietic system]
1Department of Hematology, The First Hospital of Norman Bethune Medical University, Changchun 130021, China.
Objective:
To study the hormesis effect on hematopoietic system induced by low dose radiation.
Method:
CFU-GM and BFU-E were cultured in methylcellulose semi-solid culture system, levels of GM-CSF and IL-3 were assayed by ELISA and mRNA levels of GM-CSF, G-CSF, IL-3 by in situ hybridization, narrow line hybridization and Northern blot.
Results:
(1) The in vitro yields of CFU-GM and BFU-E from radiated mice was higher than that from the control. (2) The serum protein level of GM-CSF increased obviously than that of controls; (3) mRNA levels of GM-CSF and G-CSF were also increased.
Conclusion:
There is hormesis effect on hematopoietic system induced by low dose radiation, which may be related to the increasing of cytokines.