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[8-chloroadenosine inhibits the growth factor-induced proliferation of retinal pigment epithelial cells in vitro]
1Department of Ophthalmology, The Third Hospital, Beijing Medical University, Beijing 100083.
Objective:
To study the inhibitory effect of 8-chloroadenosine (8-Cl-A) on growth factor-induced proliferation of retinal pigment epithelial (RPE) cells in vitro.
Methods:
Primary culture and subculture of bovine RPE cells were established in vitro first and then tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), basic fibroblast growth factor (bFGF) plus different concentrations of 8-Cl-A (2-16 micromol/L) were added to the cells of third passage respectively. DNA synthesis in RPE cells was measured with (3)H-TdR incorporation.
Results:
Cells treated with TNF-alpha, IL-1beta, bFGF had a higher (3)H-TdR uptake than the basal. The increase was abolished by 8-Cl-A in a dose dependent manner (P < 0.05). At TNF-alpha, IL-1beta, bFGF with 8-Cl-A group, CPM was similar to basal at 16, 8, 2 micromol/L respectively (P > 0.05).
Conclusion:
8-Cl-A can inhibit the three growth factor-induced proliferation of RPE cells.
