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Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
Published on: March 26, 2021
Inexpensive holder for hand-trimming resin-embedded tissues
1Department of Biology, University of Wisconsin-Oshkosh, Oshkosh, Wisconsin 54901, USA. wise@uwosh.edu
Microscopy Research and Technique
|March 6, 2002
Abstract:
A simple and inexpensive mount for securely holding resin-embedded tissues for trimming prior to sectioning for light or electron microscopy is described. The unit is composed of a base plate from which a 12.7-mm drill chuck protrudes. A tissue block is clamped into a microtome chuck and that is mounted in the drill chuck. A standard dissecting microscope is placed over the unit and used to magnify the block for trimming. Total cost of materials is estimated to be $20-40 USD.
