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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 29, 2026

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
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Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Published on: September 26, 2011

[Gene amplification methods in viral diagnostics]

M Lappalainen1, M Söderlund, H Piiparinen

  • 1HYKS-diagnostiikka Haartman-instituutti Helsingin yliopisto, virologian toimiala Haartmaninkatu 3, 00290 Helsinki. maija.lappalainen@helsinki.fi

Duodecim; Laaketieteellinen Aikakauskirja
|March 7, 2002
PubMed
Summary

No abstract available in PubMed .

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