[Real-time measurement of airborne pollen allergen applied the surface plasmon resonance (SPR)]

To examine the possibility of real-time measurement system for the concentration of airborne pollen allergens, Cry j 1 was chosen as an example to establish the system. Airborne pollen allergens were collected using Cyclone sampler from Burkard Co. Ltd. England. Cry j 1 was extracted from airborne samples with 10 mM HEPES buffer containing 0.125 M NH4HCO3 and measured with Biacore 3000 and Biacore Q. The sensitivity of the system was 5 ng/ml, and 0.1 ml sample volume and at least 500 pg of Cry j 1 was required for each measurement. Amounts of Cry j 1 can be obtained ca. 40 min after collection, that is, 15 min for extraction, 10 min for separation and 10 min for measurement.