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Updated: Jul 18, 2026

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In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
Published on: January 11, 2012
Quencher as leaving group: efficient detection of DNA-joining reactions
1Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Journal of the American Chemical Society
|March 7, 2002
Abstract:
We describe a new fluorescence reporting strategy in which dabsyl, a well-known quencher, activates a hydroxyl group in a probe to convert it to a leaving group. When a nucleophilic phosphorothioate probe binds adjacent to a dabsyl quenched probe, autoligation occurs, releasing the quencher, and lighting up the probes, This signal change can be used to detect single nucleotide differences in DNA without enzymes or reagents.

