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Direct on air sampling filter quantification of cat allergen.
Leif Holmquist1, Olof Vesterberg
1Respiratory Health and Climate Programme, National Institute for Working Life, Ekelundsvägen 16, Solna, S-11279 Stockholm, Sweden. olof.vesterberg@niwl.se
Journal of Biochemical and Biophysical Methods
|March 7, 2002
Summary
A new method called direct on sampling filter in solution (DOSIS) accurately quantifies airborne cat allergens. This technique revealed significantly higher allergen levels in homes with cats, highlighting potential exposure risks.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Immunology
Background:
- Airborne cat allergens, primarily Fel d 1, are significant indoor air pollutants.
- Accurate quantification of these allergens is crucial for understanding exposure and mitigating allergic reactions.
Purpose of the Study:
- To develop and validate a novel method for quantifying airborne cat allergens.
- To assess indoor air cat allergen concentrations in homes with and without cats.
Main Methods:
- Developed a direct on sampling filter in solution (DOSIS) method.
- Utilized antibody-conjugated alkaline phosphatase and chemiluminescent substrate for luminometric analysis.
- Validated precision, linearity, and limit of quantification for Fel d 1.
Main Results:
- The DOSIS method demonstrated good intra- and interassay precision.
- Quantification range was 0-100 SQ units, with a limit of 0.4 SQ units (58 pg Fel d 1).
- Homes with cats had six times higher average airborne cat allergen concentrations (142 SQ units/m³) compared to homes without (24 SQ units/m³).
Conclusions:
- The DOSIS method provides a reliable and sensitive approach for airborne cat allergen quantification.
- Airborne cat allergens are predominantly carried by large dust particles.
- Findings underscore the importance of monitoring and controlling indoor cat allergen levels.