Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cardiovascular ephrinB2 function is essential for embryonic angiogenesis.

Sebastian S Gerety1, David J Anderson

  • 1Division of Biology 216-76, California Institute of Technology, Pasadena, CA 91125, USA.

Development (Cambridge, England)
|March 7, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolutionary basis of male same-sex sexual behavior by multiple pheromone switches in Drosophila.

Current biology : CB·2026
Same author

The neural computation of affective internal states in the hypothalamus: A dynamical systems perspective.

Neuron·2025
Same author

Author Correction: Projectome-based characterization of hypothalamic peptidergic neurons in male mice.

Nature neuroscience·2025
Same author

Neural control of coordinated wing and leg movements during a terrestrial threat display.

bioRxiv : the preprint server for biology·2025
Same author

Evolutionary basis of intermale sexual behavior by multiple pheromone switches in <i>Drosophila</i>.

bioRxiv : the preprint server for biology·2025
Same author

A line attractor maintains aggressiveness during feeding in "hangry" mice.

bioRxiv : the preprint server for biology·2025
Same journal

Cell size control emerges from the vein-dependent coordinated divisions of distinct cell groups in Drosophila wing.

Development (Cambridge, England)·2026
Same journal

The people behind the papers - Kaoru Sugimura.

Development (Cambridge, England)·2026
Same journal

The people behind the papers - Zhainib Amir-Ugokwe and Kristy Red-Horse.

Development (Cambridge, England)·2026
Same journal

In preprints: toward a holistic lineage-tracing map of mammalian embryogenesis.

Development (Cambridge, England)·2026
Same journal

Endocardial TIE2 orchestrates cardiac trabeculation via sequestering FOXO1 within the cytoplasm and stimulating versican cleavage.

Development (Cambridge, England)·2026
Same journal

Mechanical interactions dynamically influence rosette morphogenesis in the migrating zebrafish Posterior Lateral Line primordium.

Development (Cambridge, England)·2026
See all related articles

EphrinB2 (EphrinB2) is crucial for blood vessel formation (angiogenesis). This study shows EphrinB2 is essential in vascular cells, not surrounding tissues, for proper blood vessel development.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cardiovascular Biology

Background:

  • EphrinB2 is a ligand for EphB receptors and is expressed in arteries.
  • EphrinB2 is implicated in angiogenesis, the formation of new blood vessels.
  • Its precise cellular requirement in angiogenesis has been debated, with potential roles in both vascular and mesenchymal cells.

Purpose of the Study:

  • To determine the specific cell type in which EphrinB2 is required for angiogenesis.
  • To investigate whether EphrinB2 in perivascular mesenchymal cells can compensate for its absence in vascular cells.

Main Methods:

  • Generation of mice with conditional deletion of EphrinB2 specifically in the endothelium and endocardium.
  • Analysis of angiogenic remodeling in these genetically modified mice.

Related Experiment Videos

  • Comparison of vascular defects with conventional EphrinB2 mutants.

    • Main Results:

    • Vascular-specific deletion of EphrinB2 resulted in complete arrest of angiogenesis.
    • The observed defects were identical to those in conventional EphrinB2 mutants.
    • EphrinB2 expression in perivascular mesenchyme did not rescue the angiogenic defects.

    Conclusions:

    • EphrinB2 is critically required in endothelial and endocardial cells for proper angiogenesis.
    • Mesenchymal EphrinB2 expression is insufficient to support vascular development in the absence of endothelial EphrinB2.
    • These findings clarify the cell-autonomous role of EphrinB2 in vascular development.