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[Involvement of FAK, PI3-K and PKC in cell adhesion induced by microtubule disruption]

Azzeddine Kadi1, Virginie Berthet, Véronique Pichard

  • 1Laboratoire de biochimie cellulaire, CNRS UMR 6032, Faculté de Pharmacie, 27, bd Jean-Moulin, 13385 Marseille Cedex 5.

Bulletin Du Cancer
|March 13, 2002
PubMed

Insights

Microtubule disruption increases cell adhesion by activating intracellular kinases, but this effect is blocked by specific inhibitors. Further research is needed to understand the exact mechanisms behind this enhanced cell attachment.

Area of Science:

  • Cell biology
  • Biochemistry
  • Molecular biology

Background:

  • Microtubule disruption is known to increase cell adhesion to extracellular matrix (ECM) proteins.
  • The precise molecular mechanisms underlying this phenomenon require further elucidation.

Purpose of the Study:

  • To investigate the intracellular signaling pathways involved in enhanced cell adhesion following microtubule disruption.
  • To identify key kinases and cellular changes associated with increased cell attachment.

Main Methods:

  • Utilized specific inhibitors of tyrosine-kinases, PI3-K (Phosphoinositide 3-kinase), and PKCs (Protein Kinase Cs).
  • Assessed tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin.
  • Examined the subcellular localization of PKC isoforms (gamma, delta, epsilon).
  • Analyzed alterations in the actin cytoskeleton and cell spreading.

Main Results:

  • Enhanced cell attachment due to microtubule depolymerization was abolished by tyrosine-kinase, PI3-K, and PKC inhibitors.
  • Microtubule depolymerization increased tyrosine phosphorylation of FAK and paxillin.
  • Specific PKC isoforms translocated to the cell interior.
  • Actin cytoskeleton changes led to reduced cell spreading.

Conclusions:

  • Microtubule depolymerization activates intracellular kinases, leading to actin cytoskeletal alterations and increased integrin-dependent cell adhesion.
  • The enhanced attachment may involve changes in integrin affinity or avidity, warranting further investigation.

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