Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antigen-induced T cell death is regulated by CD4 expression.

A R Hamad1, J P Schneck

  • 1Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

International Reviews of Immunology
|March 14, 2002
PubMed
Summary

The CD4 molecule is crucial for T cell homeostasis by regulating activation-induced cell death (AICD). Loss of CD4 prevents AICD by inhibiting FasL expression, impacting immune regulation and autoimmune disease.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomimetic biodegradable artificial antigen presenting cells synergize with PD-1 blockade to treat melanoma.

Biomaterials·2016
Same author

Novel cellular microarray assay for profiling T-cell peptide antigen specificities.

Journal of proteome research·2010
Same author

Monitoring antigen-specific T cells using MHC-Ig dimers.

Current protocols in immunology·2008
Same author

Antigen-specific blockade of T cells in vivo using dimeric MHC peptide.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same author

Brucellosis among animals and human contacts in eastern Sudan.

Saudi medical journal·2001
Same author

Lack of coreceptor allows survival of chronically stimulated double-negative alpha/beta T cells: implications for autoimmunity.

The Journal of experimental medicine·2001

Area of Science:

  • Immunology
  • Cell Biology
  • T cell biology

Background:

  • Activation-induced cell death (AICD) is vital for maintaining T cell homeostasis.
  • Fas/FasL interactions primarily mediate AICD in CD4 T cells.
  • The role of the CD4 coreceptor in TCR-dependent AICD remains unclear.

Purpose of the Study:

  • To investigate the regulatory role of the CD4 molecule in T cell activation-induced cell death.
  • To elucidate the mechanisms by which CD4 influences AICD.
  • To determine the implications of CD4's role in AICD for immune tolerance and autoimmune diseases.

Main Methods:

  • Utilized 5kc T cell hybridoma models lacking CD4 expression.
  • Assessed AICD resistance in CD4-negative T cells.
  • Investigated FasL expression levels and FasL-induced AICD.

Related Experiment Videos

  • Examined CD4 crosslinking effects on FasL expression.
  • Studied normal T cell responses to anti-CD3 and MHC/peptide stimulation.

    • Main Results:

    • Loss of CD4 rendered activated T cells resistant to AICD.
    • CD4-negative T cells exhibited inhibited FasL expression, which was reversible with recombinant FasL.
    • CD4 crosslinking independently induced FasL expression.
    • CD4 interaction with MHC/peptide complexes is critical for AICD in normal T cells.

    Conclusions:

    • The CD4 molecule plays an essential regulatory role in TCR-dependent AICD by controlling FasL expression.
    • These findings enhance understanding of peripheral tolerance mechanisms.
    • The study provides insights into the pathogenesis of autoimmune diseases.