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Updated: Jul 5, 2026

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
15:42

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Published on: March 6, 2009

Monitoring antigen-specific T cells using MHC-Ig dimers

J P Schneck1, J E Slansky, S M O'Herrin

  • 1Johns Hopkins University, Baltimore, Maryland, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed

The lack of high affinity reagents has made distinguishing T cells on the basis of antigen specificity difficult to accomplish. This unit provides protocols that utilize innovations in molecular design to permit construction of soluble multivalent MHC complexes (MHC-Ig dimers) with high avidity for cognate T cell receptors. MHC-Ig dimers display stable binding properties when they interact with antigen-specific T cells thus allowing their use in the staining of antigen-specific T cells by flow cytometry. Methods for constructing and detecting these MHC-Ig dimers are included along with protocols for applying their use for the quantitation of antigen-specific T cells.

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