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Related Experiment Video

Updated: Nov 29, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

Development of a Rapid Focus Reduction Neutralization Test Assay for Measuring SARS-CoV-2 Neutralizing Antibodies.

Abigail Vanderheiden^1,2,3, Venkata Viswanadh Edara^1,2,3, Katharine Floyd^1,2,3

¹Department of Pediatrics, Division of Infectious Disease, Emory University School of Medicine, Atlanta, Georgia.

Current Protocols in Immunology

|November 20, 2020

Summary

Researchers developed two high-throughput methods to measure neutralizing antibodies against SARS-CoV-2. These focus reduction neutralization tests (FRNT) are crucial for vaccine development and understanding COVID-19 immunity.

Keywords:

SARS-Cov-2 antibody neutralization high-throughput neutralization assay neutralizing antibodies serological assay

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Last Updated: Nov 29, 2025

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Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

Area of Science:

Virology
Immunology
Vaccinology

Background:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic with no approved vaccines.
Understanding the antibody response is critical for disease management, long-term immunity, and vaccine effectiveness.

Purpose of the Study:

To describe two novel methods for assessing the neutralization capability of antibodies against SARS-CoV-2.
To adapt these methods for high-throughput screening relevant to vaccine studies and clinical trials.

Main Methods:

A basic Focus Reduction Neutralization Test (FRNT) utilizing chromogen deposit readout.
An alternate FRNT protocol employing a fluorescent reporter (mNeonGreen) derived from an infectious clone of SARS-CoV-2 (FRNT-mNG).

Main Results:

Both described protocols provide reliable methods for quantifying SARS-CoV-2 neutralizing antibodies.
The FRNT-mNG offers a fluorescent readout adaptable for high-throughput applications.

Conclusions:

These standardized FRNT protocols are essential tools for evaluating SARS-CoV-2 vaccine candidates.
The methods facilitate large-scale studies on antibody responses crucial for combating the COVID-19 pandemic.