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Related Experiment Video

Updated: Dec 1, 2025

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Published on: March 6, 2015

Co-Culture System of Human Enteroids/Colonoids with Innate Immune Cells.

Janet F Staab¹, Jose M Lemme-Dumit², Rachel Latanich¹

¹Department of Medicine, Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Current Protocols in Immunology

|November 9, 2020

Summary

This study introduces a novel co-culture system combining human intestinal enteroids with immune cells like macrophages and neutrophils. This advanced model better mimics the gut

Keywords:

co-culture human enteroids innate immune cells intestinal organoids macrophages monolayer neutrophils

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Last Updated: Dec 1, 2025

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Published on: March 6, 2015

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Published on: March 23, 2022

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

Published on: June 30, 2021

Area of Science:

Gastroenterology and Immunology
Stem Cell Biology and Tissue Engineering
Human Gut Physiology and Disease Modeling

Background:

Human intestinal enteroids derived from adult stem cells provide an ex vivo model for gut research.
Existing enteroid models lack crucial immune cell interactions essential for intestinal homeostasis.
Innate immune cells critically influence epithelial barrier function and host defense in the gut.

Purpose of the Study:

To establish a co-culture system integrating human enteroids/colonoids with macrophages and neutrophils.
To create a more physiologically relevant in vitro model of the human intestinal epithelial niche.
To enable comprehensive study of epithelial-immune cell interactions in the gut.

Main Methods:

Enteroid/colonoid monolayers were generated from human gut biopsies.
Immune cells (macrophages, neutrophils) were isolated from human peripheral blood.
A co-culture system with separate apical and basolateral compartments was assembled.

Main Results:

A functional co-culture model of enteroids/colonoids with macrophages or neutrophils was successfully established.
Macrophage co-cultures remained viable for 48 hours; neutrophil co-cultures for 4 hours.
The system supports various outcome measures including barrier function, cytokine production, and cellular uptake.

Conclusions:

The developed co-culture system recapitulates key cellular interactions of the human intestinal niche.
This model significantly enhances the study of gut immune responses and epithelial biology ex vivo.
It offers a powerful platform for investigating gut diseases and therapeutic interventions.