Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell analysis system based on compact disk technology.

Arjan G J Tibbe1, Bart G de Grooth, Jan Greve

  • 1Biophysical Techniques Group, Faculty of Applied Physics, Twente University, PO Box 217, 7500 AE Enschede, The Netherlands. A.G.J.Tibbe@tn.utwente.nl

Cytometry
|March 14, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

WaSTE: a multicentre audit of waste segregation practices in elective knee and hip arthroplasty.

Bone & joint open·2026
Same author

Longitudinal Analysis of Manually and Automatically Classified Circulating Tumor Biomarkers and their Prediction of Survival in Metastatic Colorectal Cancer.

Clinical pharmacology and therapeutics·2025
Same author

PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients.

Cancer research communications·2025
Same author

Design, molecular characterization and therapeutic investigation of a novel CCR8 peptide antagonist that attenuates acute liver injury by inhibiting infiltration and activation of macrophages.

Acta pharmaceutica Sinica. B·2025
Same author

Performance comparison of streptavidin magnetic beads for epcam expressing cancer cell lines for circulating tumor cell (CTC) enrichment in a flow-through immunomagnetic system.

PloS one·2025
Same author

Clinical Validity of Repeated Circulating Tumor Cell Enumeration as an Early Treatment Monitoring Tool for Metastatic Breast Cancer in the PREDICT Global Pooled Analysis.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025
Same journal

Measurement of the distribution of red blood cell deformability using an automated rheoscope.

Cytometry·2002
Same journal

Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

Cytometry·2002
Same journal

Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

Cytometry·2002
Same journal

Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

Cytometry·2002
Same journal

Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

Cytometry·2002
Same journal

Separation of live cells in different phases of the cell cycle for gene expression analysis.

Cytometry·2002
See all related articles

A novel cell analysis system uses immunomagnetic alignment and CD-technology for high-sensitivity cell enumeration and differentiation. This advanced system matches or exceeds commercial flow cytometer performance without extensive sample preparation.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cell Biology

Background:

  • A new cell analysis system was developed for enumerating and differentiating magnetically aligned cells from whole blood.
  • The system's cellular information extraction is analogous to compact disk (CD) data readout.
  • Optical design and data processing of this innovative system are described.

Purpose of the Study:

  • To describe the optical design and data processing of a novel cell analysis system.
  • To demonstrate the system's performance using fluorescently labeled cells and beads.
  • To evaluate the system's sensitivity and utility for cell analysis.

Main Methods:

  • Utilized immunomagnetic cell selection and alignment.
  • Employed CD-technology for fluorescent signal analysis.

Related Experiment Videos

  • Demonstrated performance with fluorescently labeled beads and leukocytes in whole blood.

    • Main Results:

    • Achieved high sensitivity, resolving beads with as few as 333 allophycocyanin (APC) molecules, surpassing commercial flow cytometer capabilities.
    • Enabled alignment and analysis of up to 20,000 immunomagnetically labeled cells within a defined area.
    • Successfully performed a three-color CD4-CD8-CD14 assay, demonstrating system utility.

    Conclusions:

    • Developed a cell analysis system using immunomagnetic selection/alignment and CD-technology that equals or surpasses commercial analyzers.
    • The system allows for cell analysis in whole blood or other suspensions with minimal sample preparation.
    • This technology offers a powerful new tool for sensitive and efficient cell analysis.