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Related Experiment Videos

Mouse beta-mannosidase: cDNA cloning, expression, and chromosomal localization.

T Beccari1, L Bibi, S Stinchi

  • 1Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Università degli Studi di Perugia, Italy. tbeccari@unipg.it

Bioscience Reports
|March 15, 2002
PubMed
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Researchers isolated and sequenced the mouse beta-mannosidase gene, crucial for glycoprotein degradation. This gene

Area of Science:

  • Biochemistry
  • Genetics
  • Molecular Biology

Background:

  • Beta-mannosidase is an exoglycosidase essential for N-linked oligosaccharide degradation.
  • Deficiency in beta-mannosidase activity causes the lysosomal storage disorder beta-mannosidosis.

Purpose of the Study:

  • To isolate and sequence the gene encoding mouse beta-mannosidase.
  • To analyze the evolutionary conservation and tissue-specific expression of the mouse beta-mannosidase gene.

Main Methods:

  • Gene isolation and sequencing.
  • Amino acid sequence comparison across species.
  • Northern blot analysis for mRNA expression.
  • Gene mapping to mouse chromosome 3.

Main Results:

Related Experiment Videos

  • The mouse beta-mannosidase gene was successfully isolated and sequenced.
  • Mouse beta-mannosidase shares 64% amino acid identity with human, bovine, and goat orthologs, indicating high evolutionary conservation.
  • Differential mRNA expression was observed across various mouse tissues, with a major transcript of approximately 3.7 kb.
  • The mouse beta-mannosidase gene (Bmn) was mapped to the distal end of Chromosome 3, a region homologous to human Chromosome 4.

Conclusions:

  • The study provides the sequence and genomic location of the mouse beta-mannosidase gene.
  • Understanding the conservation and expression patterns aids in studying beta-mannosidosis.
  • The findings facilitate comparative genomics and research into lysosomal storage disorders.