[Study on the detection methods of Helicobacter pylori from clinical specimens]

We have attempted the detection of Helicobacter pylori using biopsy specimen (tunica mucosa vestibulum ventriculi, tunica mucosa corpus ventriculi) and of Helicobacter pylori Specific Antigen (HpSA) from feces of patients with stomach-duodenum disease. During 1991-2000, 753 patients had biopsy test for Gram-stained smear, culture, rapid urease test (RUT), and HpSA by ELISA. As a result, Gram stained smear positive were 609 (44.6%) in 1,357 specimens, culture positive were 984 (72.5%) in 1,357 specimens, and RUT positive were 445 (59.4%) in 749 specimens in method with stomach specimens. 85 (94.4%) in 90 specimens with method of HpSA were positive, and we defined that indirect detection method using feces was most significance compared with other invasive direct method using endoscopy. In addition, antimicrobial susceptibility tests were examined with 5 agents for 349 strains of H. pylori, the MICs of amoxicillin were <0.01-0.19 microgram/ml (MIC80: 0.02 microgram/ml), and clarithromicin were <0.01-50 microgram/ml (MIC80: 0.04 microgram/ml). Amoxicillin resistant strain was not observed.