Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transient, highly populated, building blocks folding model.

C J Tsai1, R Nussinov

  • 1Intramural Research Support Program, SAIC Laboratory of Experimental and Computational Biology, NCI-Frederick, MD 21702, USA.

Cell Biochemistry and Biophysics
|March 20, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loss of flavonol 3-O-glucosyltransferase activity confers soybean resistance to leaf-chewing insects.

The Plant journal : for cell and molecular biology·2026
Same author

Evaluating Speaker Introductions at the American Society for Radiation Oncology Annual Meeting.

International journal of radiation oncology, biology, physics·2021
Same author

Investigation of a Novel Decision Support Metric for Head and Neck Adaptive Radiation Therapy Using a Real-Time <i>In Vivo</i> Portal Dosimetry System.

Technology in cancer research & treatment·2019
Same author

Mutations in LZTR1 drive human disease by dysregulating RAS ubiquitination.

Science (New York, N.Y.)·2018
Same author

Agrobacterium-mediated transformation of quaking aspen (Populus tremuloides) and regeneration of transgenic plants.

Plant cell reports·2013
Same author

Contribution of salt bridges toward protein thermostability.

Journal of biomolecular structure & dynamics·2012
Same journal

From Isoprene Units to Polyprenols and Dolichols: 70 Years of Polyisoprenoid Biosynthesis Research.

Cell biochemistry and biophysics·2026
Same journal

Therapeutic Insights into the Hepatoprotective Effect of Strobilanthes callosa.

Cell biochemistry and biophysics·2026
Same journal

The Dual Role of TGF-β and Hypoxia on MMP14-Mediated Invasion in PC3 Cells.

Cell biochemistry and biophysics·2026
Same journal

Microalgae-Mediated Synthesis of Gold Nanoparticles from Indonesian Chlorella vulgaris InaCC M205 with Potential Anticancer Properties for Biomedical Application.

Cell biochemistry and biophysics·2026
Same journal

Adsorption-driven Graphene Oxide SPR Biointerfaces: Coupled Thermodynamic, Kinetic, and Optical Modeling with Effective-layer Validation.

Cell biochemistry and biophysics·2026
Same journal

Identification of Ferroptosis-related Genes Associated with the Prognosis of Hepatocellular Carcinoma.

Cell biochemistry and biophysics·2026
See all related articles

Protein folding initiates from local structural elements, assembling into complex native conformations. This hierarchical model explains protein folding pathways and kinetics, consistent with experimental data.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Computational Biology

Background:

  • Protein folding is a complex process involving the hierarchical assembly of local structural elements into a native conformation.
  • Multiple folding pathways exist, but only a few are predominantly utilized.

Purpose of the Study:

  • To present a hierarchical, building block model for protein folding.
  • To contextualize this model within existing experimental and theoretical findings.
  • To explore the model's utility in understanding folding pathways, chaperonin assistance, and domain swapping.

Main Methods:

  • Review of existing literature on protein folding.
  • Presentation of a hierarchical, building block protein-folding model based on two premises.
  • Algorithmic description and illustration of the model's application.

Related Experiment Videos

  • Analysis of folding pathways, chaperonin-assisted folding, domain-swapping, disconnectivity graphs, and tree diagrams.

    • Main Results:

    • The model posits that protein folding progresses via combinatorial assembly of transient local structural elements.
    • Binding of favorable conformers drives the folding reaction by redistributing solution conformers.
    • Early sequential interactions are likely, even for complex nonsequential native folds, offering kinetic and entropic advantages.
    • The model enables derivation of major folding pathways and qualitatively explains folding kinetics.

    Conclusions:

    • Protein folding initiated from local transient structural elements aligns with experimental and theoretical data.
    • The hierarchical building block model provides a framework for understanding the kinetics and pathways of protein folding.
    • The model's ability to derive folding pathways and explain kinetics supports its utility in structural biology.