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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Fluorescent pteridine nucleoside analogs: a window on DNA interactions.

M E Hawkins1

  • 1Pediatric Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USA. mh100x@nih.gov

Cell Biochemistry and Biophysics
|March 20, 2002
PubMed
Summary

Pteridine nucleoside analogs are fluorescent probes that can be incorporated into DNA to detect molecular interactions. These probes offer sensitive, real-time monitoring of DNA structural changes through fluorescence measurements.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysical Chemistry

Background:

  • Pteridine nucleoside analogs are highly fluorescent molecules.
  • Their structural similarity to native nucleosides allows incorporation into oligonucleotides.
  • These probes can be used to monitor DNA interactions with other molecules.

Purpose of the Study:

  • To review the fluorescence properties of pteridine nucleoside analogs.
  • To detail specific guanosine (3MI, 6MI) and adenosine (6MAP, DMAP) analogs.
  • To explore applications of these probes in studying DNA interactions.

Main Methods:

  • Incorporation of pteridine analogs into oligonucleotides via automated DNA synthesis using phosphoramidite chemistry.
  • Measurement of fluorescence properties including intensity, anisotropy, lifetimes, spectral shifts, and energy transfer.
  • Analysis of pteridine analogs as monomers and within single- and double-stranded oligonucleotides.

Main Results:

  • Pteridine nucleoside analogs exhibit distinct fluorescence characteristics.
  • Incorporation into oligonucleotides yields probes sensitive to structural alterations.
  • The fluorescence of these probes changes measurably upon interaction with other molecules.

Conclusions:

  • Pteridine nucleoside analogs are versatile tools for studying DNA-molecule interactions.
  • Their fluorescence-based detection provides sensitive insights into structural dynamics.
  • Specific analogs like 3MI, 6MI, 6MAP, and DMAP have demonstrated utility in various applications.