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Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...

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Related Experiment Video

Updated: Jun 27, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Rescuing the function of mutant p53.

A N Bullock1, A R Fersht

  • 1Department of Biochemistry, University of Washington, Seattle, USA.

Nature Reviews. Cancer
|March 20, 2002
PubMed
Summary
This summary is machine-generated.

The tumor suppressor protein p53 is crucial for cancer prevention, but mutations can inactivate it. Future cancer drugs may aim to restore p53

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Last Updated: Jun 27, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

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Published on: August 4, 2019

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10:55

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Published on: December 30, 2025

Area of Science:

  • Oncology
  • Molecular Biology
  • Protein Biochemistry

Background:

  • The tumor suppressor protein p53 is a critical cellular component that prevents cancer development.
  • p53 functions by inducing cell death or cell cycle arrest in cells with damaged DNA.
  • The therapeutic efficacy of p53 is contingent upon its native, intact three-dimensional structure.

Purpose of the Study:

  • To explore therapeutic strategies for restoring the function of mutated p53 in cancer.
  • To investigate methods for stabilizing the native conformation of the p53 protein.
  • To identify potential drug targets that can reactivate p53's tumor-suppressive activities.

Main Methods:

  • Analysis of p53 mutations identified in cancer patients.
  • Computational modeling to understand p53 protein structure and stability.
  • In vitro and in vivo assays to evaluate the effects of potential therapeutic interventions.

Main Results:

  • Identified numerous p53 mutations that compromise its native conformation and function.
  • Demonstrated that single nucleotide changes can lead to significant loss of p53 activity.
  • Highlighted the challenge of restoring p53 function due to its structural fragility.

Conclusions:

  • p53's critical role in cancer prevention is often subverted by mutations affecting its structure.
  • Developing drugs that can stabilize or restore the native p53 conformation is a promising therapeutic avenue.
  • Further research is needed to identify and develop effective p53-restoring cancer therapies.