Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pre-instrumental variables in coagulation testing.

J A Koepke, J L Rodgers, M J Ollivier

    American Journal of Clinical Pathology
    |November 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Corrections For Spurious Influences On Correlations Between Mmpi Scales.

    Multivariate behavioral research·2016
    Same author

    The Bootstrap, the Jackknife, and the Randomization Test: A Sampling Taxonomy.

    Multivariate behavioral research·2016
    Same author

    A study of roder ectoparasites in Mobile, Ala.

    Public health reports (Washington, D.C. : 1896)·2010
    Same author

    Epdiemiological significance of seasonal variations in rodent-ectoparasite distribution.

    Public health reports (Washington, D.C. : 1896)·2010
    Same author

    International Council for Standardization in Haematology (ICSH) recommendations for "surrogate reference" method for the packed cell volume.

    Laboratory hematology : official publication of the International Society for Laboratory Hematology·2003
    Same author

    Seasonality of birth in nineteenth- and twentieth-century Austria.

    Social biology·2002
    Same journal

    Invasive urothelial carcinoma in association with surface low-grade papillary urothelial carcinoma: clinical and pathologic insights from a rare entity.

    American journal of clinical pathology·2026
    Same journal

    CEACAM1 expression by immunohistochemistry in B-cell lymphomas and plasma cell myeloma.

    American journal of clinical pathology·2026
    Same journal

    Comprehensive multicriteria life cycle assessment of biopsy processing in a surgical pathology department.

    American journal of clinical pathology·2026
    Same journal

    Prognostic significance of Myb protein and its downstream target genes in lacrimal gland adenoid cystic carcinoma.

    American journal of clinical pathology·2026
    Same journal

    Mismatch repair protein "nonclassic expression loss" pattern in colorectal cancer: an important staining pattern that is not well understood.

    American journal of clinical pathology·2026
    Same journal

    Musculoskeletal pain in medical laboratory personnel: a cross-sectional study.

    American journal of clinical pathology·2026
    See all related articles

    To improve accuracy in coagulation testing, a new blood anticoagulation ratio is proposed for polycythemia patients. This adjustment ensures reliable prothrombin time (PT) and partial thromboplastin time (PTT) results, even with sample storage.

    Area of Science:

    • Clinical Chemistry
    • Hematology
    • Diagnostic Laboratory Medicine

    Background:

    • Accurate measurement of prothrombin time (PT) and partial thromboplastin time (PTT) is crucial for diagnosing and monitoring coagulation disorders.
    • Standard anticoagulation protocols may yield inaccurate results in patients with specific hematological conditions like polycythemia.

    Purpose of the Study:

    • To investigate variables affecting PT and PTT measurements.
    • To establish an optimized anticoagulation method for blood samples from patients with polycythemia.
    • To assess the stability of blood specimens under various storage conditions.

    Main Methods:

    • Systematic examination of factors influencing PT and PTT.
    • Comparative analysis of anticoagulation ratios using sodium citrate solution.

    Related Experiment Videos

  • Evaluation of specimen stability at room temperature over time (up to 24 hours).

    • Main Results:

    • A proposed anticoagulation ratio of 1:19 (3.8% sodium citrate to whole blood) minimizes excessive anticoagulation in polycythemic samples.
    • This adjusted ratio prevents spuriously prolonged PT and PTT results in polycythemia without negatively impacting anemic samples.
    • Unopened, vacuum-drawn specimens show no significant deterioration within 6 hours at room temperature.
    • Prothrombin time measurements remain stable for up to 24 hours.
    • Partial thromboplastin time shows a 10-15% increase after 24 hours of storage.

    Conclusions:

    • The recommended 1:19 anticoagulation ratio is effective for accurate PT and PTT testing in polycythemia patients.
    • Optimized anticoagulation improves diagnostic reliability for coagulation tests.
    • Understanding specimen stability is vital for interpreting PT and PTT results, with PTT being more sensitive to prolonged storage.