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Updated: Mar 20, 2026
10:23
High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
Published on: April 20, 2017
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Endoplasmic reticulum dynamics, inheritance, and cytoskeletal interactions in budding yeast
K L Fehrenbacher1, D Davis, M Wu
1Department of Anatomy and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA.
Molecular Biology of the Cell
|March 22, 2002
Summary
This study reveals distinct mechanisms for endoplasmic reticulum (ER) inheritance in yeast. Microtubules mediate nuclear ER inheritance, while actin and anchorage govern cortical ER inheritance.
Area of Science:
- Cell Biology
- Molecular Biology
- Cytoskeletal Dynamics
Background:
- The endoplasmic reticulum (ER) in Saccharomyces cerevisiae comprises cortical and nuclear components.
- Understanding the inheritance mechanisms of these distinct ER structures is crucial for cell division.
Purpose of the Study:
- To investigate the motility and inheritance mechanisms of cortical and nuclear ER in living yeast cells.
- To elucidate the roles of cytoskeletal elements in ER inheritance.
Main Methods:
- Utilized a Sec63p-green fluorescent protein fusion to visualize ER dynamics in vivo.
- Observed ER motility during different cell cycle phases (M, S, G2).
- Investigated the effects of microtubule and actin cytoskeleton disruption on ER inheritance.
Main Results:
- Nuclear ER exhibits microtubule-dependent extension and movement during M phase.
- Cortical ER shows anchorage-dependent inheritance at bud emergence sites.
- Actin cytoskeleton and ACT1 gene are critical for cortical ER morphology and inheritance.
Conclusions:
- Nuclear and cortical ER inheritance involve different mechanisms and cytoskeletal mediators.
- Cortical ER inheritance is cytoskeleton-dependent, relying on anchorage rather than directed movement.
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