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Studies on the multiple forms of gamma-glutamyltransferase
Clinica Chimica Acta; International Journal of Clinical Chemistry
|December 1, 1976
Summary
Researchers investigated elevated gamma-glutamyltransferase (gamma-GT) levels in patients. They found that gamma-GT separation into two fractions is linked to lipoprotein distribution, not true isoenzymes.
Area of Science:
- Biochemistry
- Clinical Chemistry
Background:
- Elevated gamma-glutamyltransferase (gamma-GT) activity is a common clinical finding.
- Understanding the nature of gamma-GT fractions can aid in disease diagnosis and monitoring.
Purpose of the Study:
- To investigate the cause of gamma-GT separation into distinct fractions in patient sera.
- To determine if these fractions represent true isoenzymes or are influenced by other serum components.
Main Methods:
- Sera from 53 patients with high gamma-GT activity were analyzed.
- Column chromatography using Sephadex DEAE A-50 was employed for gamma-GT separation.
- Additional biochemical parameters, including lipoproteins and cholesterol, were examined in a subset of samples.
Main Results:
- In 35 sera, a single gamma-GT fraction was observed at 2.0--2.5 mS conductivity.
- In 18 sera, two gamma-GT fractions were detected at 0.2 mS and 2.0--2.5 mS conductivity.
- The separation into two fractions was attributed to varying distributions of cholesterol-containing lipoproteins, not true isoenzymes.
Conclusions:
- The observed separation of gamma-glutamyltransferase into two fractions is not due to true isoenzymes.
- Lipoprotein distribution significantly influences the chromatographic behavior of gamma-GT.
- This finding has implications for the interpretation of gamma-GT assays in clinical settings.