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Subcellular localization of the yeast proteome.
Anuj Kumar1, Seema Agarwal, John A Heyman
1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Genes & Development
|March 27, 2002
Summary
This study mapped the location of nearly all proteins in yeast, revealing their cellular functions and identifying many previously uncharacterized proteins. The yeast localizome data aids in understanding eukaryotic protein function.
Area of Science:
- Cell Biology
- Proteomics
- Yeast Genetics
Background:
- Protein localization is crucial for understanding eukaryotic protein function.
- Previous studies lacked proteome-scale localization data for any eukaryote.
Purpose of the Study:
- To perform the first proteome-scale analysis of protein localization in Saccharomyces cerevisiae.
- To define the yeast localizome and identify proteins of unknown function.
Main Methods:
- Epitope-tagging of 60% of the yeast proteome using directed cloning and transposon mutagenesis.
- High-throughput immunolocalization to determine subcellular localization of 2744 proteins.
- Bayesian computational analysis to extrapolate data to the entire yeast proteome.
Main Results:
- Defined the yeast localizome, estimating 5100 soluble and >1000 transmembrane proteins.
- Determined the distribution: 47% cytoplasmic, 13% mitochondrial, 13% exocytic, 27% nuclear/nucleolar.
- Provided localization data for 955 proteins of previously unknown function.
Conclusions:
- The yeast localizome provides a comprehensive resource for understanding protein function and cellular organization.
- Localization data significantly aids in characterizing functionally uncharacterized proteins.
- Nuclear proteins are disproportionately essential for spore viability.