Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exploring the living cochlea using confocal microscopy.

Mats Ulfendahl1, Jacques Boutet de Monvel, Sophie Le Calvez

  • 1Institute for Hearing and Communication Research and Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden. mats.ulfendahl.ihk.ki.se

Audiology & Neuro-Otology
|March 27, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic Medicine Sweden: Advancing precision medicine at the national level.

Journal of internal medicine·2026
Same author

Deep-learning based morphological segmentation of canine diffuse large B-cell lymphoma.

Frontiers in veterinary science·2025
Same author

The digital revolution in veterinary pathology.

Journal of comparative pathology·2024
Same author

A free intravesicular C-terminal of otoferlin is essential for synaptic vesicle docking and fusion at auditory inner hair cell ribbon synapses.

Progress in neurobiology·2024
Same author

In utero adeno-associated virus (AAV)-mediated gene delivery targeting sensory and supporting cells in the embryonic mouse inner ear.

PloS one·2024
Same author

A Preliminary Study Assessing a Transfer Learning Approach to Intestinal Image Analysis to Help Determine Treatment Response in Canine Protein-Losing Enteropathy.

Veterinary sciences·2024

This study visualizes inner ear cellular structures using advanced confocal microscopy. Image restoration techniques improve visualization of the organ of Corti for studying cellular events.

Area of Science:

  • Oto-neuroscience
  • Cellular Biology
  • Microscopy

Background:

  • Understanding cochlear cellular behavior requires visualizing structures below the reticular lamina.
  • High-resolution imaging of the intact organ of Corti is crucial for studying cellular events.

Purpose of the Study:

  • To develop and apply advanced imaging techniques for high-resolution visualization of inner ear cellular structures.
  • To investigate cellular events, such as structural reorganization after acoustic overstimulation, within the intact organ of Corti.

Main Methods:

  • Utilized confocal microscopy on in vitro preparations of guinea pig and mouse inner ears.
  • Applied wavelet denoising and deconvolution algorithms for significant image restoration of confocal microscopy data.

Related Experiment Videos

Main Results:

  • Achieved high-resolution visualization of cellular structures within the intact organ of Corti.
  • Demonstrated that image restoration techniques can overcome limitations of confocal microscopy, such as light scattering and low signal-to-noise ratio.

Conclusions:

  • Confocal microscopy combined with image restoration offers a powerful approach to study detailed cellular events in the inner ear.
  • Future research will focus on dynamic cellular events and in vivo models for enhanced functional visualization.