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Related Experiment Videos

A sampling and analytical methodology for dental trace element analysis.

Catriona J Brown1, Simon R N Chenery, Barry Smith

  • 1Eastman Dental Institute, London, UK.

The Analyst
|March 28, 2002
PubMed
Summary
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Trace element analysis in primary teeth offers insights into environmental health. Country of origin significantly impacts tooth elemental composition, but age and gender do not when tooth type is controlled.

Area of Science:

  • Environmental Health
  • Human Nutrition
  • Analytical Chemistry

Background:

  • Trace elements are crucial for human health and linked to environmental pollution.
  • Primary teeth serve as valuable bio-monitors for assessing environmental exposures and nutritional status.
  • Understanding elemental composition in teeth requires robust sampling and analytical methods.

Purpose of the Study:

  • To develop a sampling and analytical strategy for determining trace element content in primary teeth.
  • To evaluate the utility of primary teeth in environmental health and nutrition studies.
  • To investigate the influence of various factors on tooth elemental composition.

Main Methods:

  • Collection of exfoliated and extracted primary teeth from children in Uganda and the UK.

Related Experiment Videos

  • Separation of tooth crown and root, followed by digestion and analysis using Inductively Coupled Plasma Mass Spectrometry (ICP-MS).
  • Statistical analysis including Principal Components Analysis (PCA) to assess the influence of country, tooth type, age, and gender, and to manage outliers.

    • Main Results:

    • Country of origin significantly influences the elemental composition of primary teeth.
    • Tooth type (crown vs. root) must be controlled in elemental analysis studies.
    • Age and gender were found to have no significant effect on elemental composition when tooth type was standardized.
    • Elemental concentrations within a standardized tooth type were representative of an individual, potentially indicating health status.

    Conclusions:

    • Primary teeth are effective bio-indicators for environmental exposure and health status.
    • Standardized methods controlling for country and tooth type are essential for reliable trace element analysis.
    • This approach provides a foundation for using deciduous teeth in large-scale environmental and nutritional health assessments.