Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiplex Pyrosequencing.

Nader Pourmand1, Elahe Elahi, Ronald W Davis

  • 1Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA.

Nucleic Acids Research
|March 28, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scalable longitudinal imaging and transcriptomics of cells in dynamic enclosures.

bioRxiv : the preprint server for biology·2026
Same author

Noninvasive methods to monitor dynamic single-cell events.

Trends in cell biology·2026
Same author

Benchmarking long-read assembly tools and preprocessing strategies for bacterial genomes: A case study on <i>E. coli</i> DH5α.

Biotechnology reports (Amsterdam, Netherlands)·2025
Same author

GDAP1-Related Charcot-Marie-Tooth Disease: Axonal or Demyelinating Subtype? Autosomal Recessive or Autosomal Dominant Inheritance?

Journal of the peripheral nervous system : JPNS·2025
Same author

Atypical presentations in an RTD patient and report of novel SLC52A3 and SLC52A2 mutations.

Acta neurologica Belgica·2024
Same author

Identification of a mutation in <i>TNRC18</i> in a patient with clinical features of Fazio-Londe disease.

Clinical case reports·2024
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

A novel single-tube multiplex Pyrosequencing assay simplifies genetic analysis. This method enables efficient single nucleotide polymorphism genotyping and microbial typing using PCR amplification and sequencing.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Pyrosequencing is a valuable technique for DNA sequencing.
  • Multiplexing assays can increase throughput and efficiency.
  • A simplified, single-tube approach is desirable for broader application.

Purpose of the Study:

  • To develop a novel, simple, single-tube multiplex Pyrosequencing assay.
  • To demonstrate its utility for genetic analysis applications.

Main Methods:

  • Genomic DNA or cDNA was PCR amplified using biotinylated and normal primers.
  • PCR products were captured on streptavidin-coated beads, followed by single-stranded DNA separation.
  • Multiplex Pyrosequencing was performed using multiple sequencing primers.

Related Experiment Videos

Main Results:

  • The assay generated unique pyrogram patterns based on nucleotide incorporation signal intensity.
  • Demonstrated successful application for single nucleotide polymorphism (SNP) genotyping.
  • Showcased utility for microbial typing.

Conclusions:

  • The developed single-tube multiplex Pyrosequencing assay is a simple and effective method.
  • It offers a streamlined approach for SNP genotyping and microbial identification.
  • This assay has potential for various genetic analysis and diagnostic applications.