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Published on: March 26, 2018
Gene expression in CD34(+) cells from normal bone marrow and leukemic origins
1Chinese National Human Genome Center at Shanghai, Shanghai 201203, PR China.
Summary
Researchers cataloged gene expression in normal bone marrow and myelodysplastic syndrome-acute myeloid leukemia (MDS-AML) CD34(+) cells. Distinct gene patterns in MDS-AML cells may drive leukemia development and maintenance.
Area of Science:
- Hematology
- Molecular Biology
- Genomics
Background:
- Hematopoiesis regulation and leukemogenesis are complex.
- Understanding gene expression in hematopoietic stem cells is crucial.
Purpose of the Study:
- To establish gene expression catalogs for normal bone marrow and leukemia CD34(+) cells.
- To identify differentially expressed genes in myelodysplastic syndrome-acute myeloid leukemia (MDS-AML).
Main Methods:
- Constructed CD34(+) cell cDNA libraries from normal bone marrow and MDS-AML.
- Generated expressed sequence tags (ESTs) and full-length cDNAs.
- Utilized bioinformatic tools for sequencing and annotation.
Main Results:
- Cataloged thousands of ESTs and identified numerous known and novel genes.
- Found 58 transcripts with statistically significant differences in copy numbers between normal and MDS-AML cells.
- Cloned 20 novel full-length cDNAs from MDS-AML cells.
Conclusions:
- MDS-AML CD34(+) cells exhibit distinct gene expression profiles compared to normal cells.
- These unique gene expression patterns may contribute to the development and progression of leukemia.
