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Updated: Oct 2, 2026

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
Mass spectrometric analysis of cyclofenil and its metabolites in human urine
Seung-Woon Myung1, Hye-Ki Min, Dong-Hyun Kim
1Doping Control Center, Korea Institute of Science and Technology, P.O. Box 131, Seoul 136-791, Korea. swmyung@kist.re.kr
Abstract:
Using gas chromatography/electron impact-mass spectrometry (GC/EI-MS) and high performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI-MS/MS), the structures of cyclofenil metabolites in human urine have been assigned. The hydroxyl metabolites liberated from the glucuronide conjugates after acid hydrolysis were characterized as the trimethylsilyl (O-TMS) derivatives using GC/MS. The conjugate glucuronide forms were detected without hydrolysis by HPLC/MS. Cyclofenil was not observed in urine. Tentative structures for the two metabolites are proposed.
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