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Related Experiment Videos

Nerve growth factor selectively regulates expression of transcripts encoding ribosomal proteins.

James M Angelastro1, Béata Töröcsik, Lloyd A Greene

  • 1Department of Pathology and Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, 630 W, 168th Street, New York, NY 10032, USA. jma14@columbia.edu

BMC Neuroscience
|April 2, 2002
PubMed
Summary

Nerve growth factor (NGF) selectively alters ribosomal protein gene expression in PC12 cells. This regulation, observed via Serial Analysis of Gene Expression (SAGE), impacts neuronal differentiation and cell cycle withdrawal.

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Area of Science:

  • Molecular Biology
  • Neuroscience
  • Gene Expression Analysis

Background:

  • Nerve growth factor (NGF) promotes neuronal differentiation and survival.
  • PC12 cells are a valuable model for studying NGF mechanisms, including gene expression regulation.
  • Serial Analysis of Gene Expression (SAGE) was employed to analyze transcript levels.

Purpose of the Study:

  • To investigate the effect of NGF on ribosomal protein transcript levels in PC12 cells.
  • To identify specific ribosomal protein transcripts regulated by NGF.
  • To compare NGF-regulated ribosomal protein transcript expression with overall gene expression changes.

Main Methods:

  • Utilized SAGE to quantify approximately 22,000 transcripts in PC12 cells.
  • Identified 74 transcripts encoding ribosomal proteins from SAGE libraries.

Related Experiment Videos

  • Analyzed NGF-induced changes in the relative abundance of ribosomal protein transcripts.

    • Main Results:

    • Ribosomal protein transcripts constituted a significant portion (3.5-5.2%) of total transcripts.
    • Nearly half of detected ribosomal protein transcripts showed statistically significant alterations in response to NGF.
    • Approximately two-thirds of these changes involved decreased transcript abundance, with some up to 5-fold.
    • RPL9 transcript levels increased within 1 hour and peaked by 8 hours post-NGF treatment.

    Conclusions:

    • NGF selectively modulates the expression of ribosomal protein transcripts.
    • These findings suggest a role for ribosomal protein transcript regulation in NGF-mediated neuronal differentiation and cell cycle exit.
    • Regulation of individual ribosomal protein transcripts is specific to cell type and stimulus.